Abstract: The wheel-rail forces of 20 normal-operating trains passing through rails with upper locking double-layer non-linear turnout fasteners were tested on-site at the Metro and the data analyzed. The results showed when a train passed a double-layer non-linear turnout fastener, the maximum wheel load reduction rate was 0.14, the maximum derailment coefficient was 0.41, the average vertical force of the left and right wheels was 57.6 kN, and the average lateral force was 15.8 kN, which showed that the locking double-layer non-linear turnout fasteners on the upper part of the depot could fully meet the safety indicators of train operation.